Earth’s Cryosphere, 2019, Vol. XXIII, No. 6, p. 31-38
DIVERSITY OF CULTURED PROKARYOTES IN PERMAFROST SEDIMENT SAMPLES FROM WEST SPITSBERGEN ISLAND
V.E. Trubitsyn1, Y.V. Ryzhmanova1, A.G. Zaharuk1, V.I. Oshurkova1, K.S. Laurinavichius1, E.V. Spirina2, V.A. Shcherbakova1, E.M. Rivkina2
1 Skryabin Institute of Biochemistry and Physiology of Microorganisms, RAS,
5, prosp. Nauki, Moscow region, Pushchino, 142290, Russia; lichoradkin43@gmail.com
2 Institute of Physicochemical and Biological Problems in Soil Science, RAS,
2, Institutskaya str., Moscow region, Pushchino, 142290, Russia
The diversity of anaerobic and aerobic microorganisms (prokaryotes) was studied in permafrost soil sampled at a depth of 0.5 to 3.7 m during the 2016 expedition of the State Scientific Centre of the Russian Federation the Arctic and Antarctic Research Institute (AARI). The estimated number of organotrophic microorganisms varies from 3.29•10 to 7.0•104 CFU•g–1 for aerobic, and from 3.0•10 to 2.3•104 cell•g–1 for anaerobic organisms. In separate anaerobically cultivated samples, methane and acetate were observed, while sulfate and iron reducing prokaryotes were detected in none of the samples. In the course of research, 60 strains of aerobic psychrophilic and psychotolerant bacteria were isolated. The taxonomic position of the isolated microorganisms was established by sequencing the 16S rRNA genes and using MALDI mass spectrometry. The created collection of strains consisted of representatives of the Actinobacteria, Firmicutes, Betaproteobacteria and Gammaproteobacteria phyla.
Permafrost sediments, West Spitsbergen, microbial communities, psychrophilic microorganisms, anaerobic prokaryotes